A feasibility study fornoninvasive measurement of shear wave speed in live zebrafish.

Zebrafish are being increasingly used as animal models for human diseases such as cardiomyopathy and neuroblastoma. Owing to a nearly fully sequenced genome and efficient genetics/chemical genetics, zebrafish open new research opportunities for human diseases research. The purpose of this study was to develop zebrafish ultrasound vibro-elastography (ZUVE) for measuring the shear wave speed of zebrafish.

An adult female zebrafish was anesthetized for three minutes for the ZUVE testing. A 0.1 s gentle harmonic vibration was generated on the tail using a sphere tip indenter with 3 mm diameter. Shear wave propagation in the zebrafish was measured using a high frequency 18 MHz ultrasound probe. Shear wave speeds were measured at 300, 400, and 500 Hz.

Individual Reaction Mix 9
G065-9	ABM	200 reactions	EUR 167

7095D 9 PASTEUR PIPET BOROSILICATE
7095D-9	CORNING	200/pk	EUR 78
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Bovine AGP ELISA Kit
E-10AGP	Immunology Consultants Laboratory	1 x 96 well plate	EUR 461

Rat AGP ELISA Kit
E-25AGP	Immunology Consultants Laboratory	1 x 96 well plate	EUR 441

Chicken AGP ELISA Kit
E-30AGP	Immunology Consultants Laboratory	1 x 96 well plate	EUR 441

Dog AGP ELISA Kit
E-40AGP	Immunology Consultants Laboratory	1 x 96 well plate	EUR 492

Human AGP ELISA Kit
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Human Brain Tissue Preparation Buffer 9: Normal Nerve Microglia
9-80021	CHI Scientific	1 x 100 ml	Ask for price

Human Cancer Tissue Preparation Buffer 9: Kidney Tumor Cells
9-80032	CHI Scientific	1 x 100 ml	Ask for price

Mouse Brain Tissue Preparation Buffer 9: Normal Nerve Microglia
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Sheep A1-AGP ELISA Kit
ESA0695	Abclonal	96Tests	EUR 521

Mouse A1-AGP ELISA Kit
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Monkey A1-AGP ELISA Kit
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Porcine A1-AGP ELISA Kit
EPA0695	Abclonal	96Tests	EUR 521

Rat A1-AGP ELISA Kit
ERA0695	Abclonal	96Tests	EUR 521

Rabbit A1-AGP ELISA Kit
ERTA0695	Abclonal	96Tests	EUR 521

Anserini A1-AGP ELISA Kit
EAA0695	Abclonal	96Tests	EUR 521

Goat A1-AGP ELISA Kit
EGTA0695	Abclonal	96Tests	EUR 521

Canine A1-AGP ELISA Kit
ECA0695	Abclonal	96Tests	EUR 521

Chicken A1-AGP ELISA Kit
ECKA0695	Abclonal	96Tests	EUR 521

Human A1-AGP ELISA Kit
EHA0695	Abclonal	96Tests	EUR 521

Bovine A1-AGP ELISA Kit
EBA0695	Abclonal	96Tests	EUR 521

AGP ELISA Kit (Bovine) (OKCD06846)
OKCD06846	Aviva Systems Biology	96 Wells	EUR 1040
Description: Description of target: Functions as transport protein in the blood stream.UniRule annotation;Species reactivity: Bovine;Application: ELISA;Assay info: ;Sensitivity: < 7.0ng/mL

Human Angiotensin 1-9 (Ang1-9) ELISA Kit
DLR-Ang1-9-Hu-48T	DL Develop	48T	EUR 501
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Human Angiotensin 1-9 (Ang1-9) ELISA Kit
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Human Angiotensin 1-9 (Ang1-9) ELISA Kit
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Human Angiotensin 1-9 (Ang1-9) ELISA Kit
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Human Carbohydrate Antigen 19-9 (CA19-9) ELISA Kit
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Human Carbohydrate Antigen 19-9 (CA19-9) ELISA Kit
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Human Carbohydrate Antigen 19-9 (CA19-9) ELISA Kit
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Human Carbohydrate Antigen 19-9 (CA19-9) ELISA Kit
RDR-CA19-9-Hu-48Tests	Reddot Biotech	48 Tests	EUR 455

Human Carbohydrate Antigen 19-9 (CA19-9) ELISA Kit
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Guinea Pig A1-AGP ELISA Kit
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Alpha 1 Acid Glycoprotein (A1-AGP), bovine
A1AG20-N	Alpha Diagnostics	1 mg	EUR 164

Alpha 1 Acid Glycoprotein (A1-AGP), Rabbit
A1AG23-N-25	Alpha Diagnostics	25 ug	EUR 286

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A1AG24-N-100	Alpha Diagnostics	100 ug	EUR 164

anti-α1-acid glycoprotein (AGP)(29A1)
LF-MA0167	Abfrontier	100 ul	EUR 334
Description: Mouse monoclonal to α1-acid glycoprotein (AGP)

Porcine α1-Acid glycoprotein,α1-AGP ELISA Kit
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Porcine α1-Acid glycoprotein,α1-AGP ELISA Kit
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Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit
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Human α1-Acid glycoprotein(α1-AGP)ELISA Kit
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Alpha 1 Acid Glycoprotein (A1-AGP), Human Plasma
A1AG15-N	Alpha Diagnostics	1 mg	EUR 164

Mouse α1-AGP(α1-Acid glycoprotein) ELISA Kit
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Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit
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Mouse α1-Acid glycoprotein(α1-AGP)ELISA Kit
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Human α1-Acid glycoprotein(α1-AGP)ELISA Kit
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Bovine α1-Acid glycoprotein,α1-AGP ELISA Kit
CN-00487B1	ChemNorm	96T	EUR 458

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A1AG22-N-100	Alpha Diagnostics	100 ug	EUR 164

Human Alpha-1-Acid Glycoprotein ELISA Kit (AGP 1)
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QY-E80114	Qayee Biotechnology	96T	EUR 426

AQP1-AQP9 and AQPAP control/blocking peptides (25 ug each of AQP1-9)
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Rabbit Anti-AQP1-AQP9 and AQPAP Antiserum (25 ul each of AQP1-9)
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ELISA kit for Rat ?1-AGP (Alpha-1-Acid Glycoprotein)
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Description: A sandwich ELISA kit for quantitative measurement of Rat ?1-AGP (Alpha-1-Acid Glycoprotein) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Human ?1-AGP (Alpha-1-Acid Glycoprotein)
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Human Alpha-1-Acid Glycoprotein (Orosomucoid, AGP) AssayMax ELISA Kit
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Human Alpha-1-Acid Glycoprotein (Orosomucoid, AGP) AssayMax ELISA Kit
EG5101-1	AssayPro	96 Well Plate	EUR 396

Human Alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody (Biotin Conjugate)
12011-05021	AssayPro	150 ug	EUR 276

Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody (Biotin Conjugate)
32220-05121	AssayPro	150 ug	EUR 369

Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit
CSB-E12730r-24T	Cusabio	1 plate of 24 wells	EUR 165
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1-CSB-E12730r	Cusabio	EUR 703.00 EUR 4843.00 EUR 2570.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
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1-CSB-E08579b	Cusabio	EUR 703.00 EUR 4843.00 EUR 2570.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
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20-abx121993	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul

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abx231218-100ug	Abbexa	100 ug	EUR 481

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abx233223-100ug	Abbexa	100 ug	EUR 481

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RC212-20	Bio Basic	10ug	EUR 169.63

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GT15020	Neuromics	100 ug	EUR 526

Human Alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) AssayLite Antibody (FITC Conjugate)
12011-05041	AssayPro	150 ug	EUR 428

Human Alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) AssayLite Antibody (RPE Conjugate)
12011-05051	AssayPro	150 ug	EUR 428

Human Alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) AssayLite Antibody (APC Conjugate)
12011-05061	AssayPro	150 ug	EUR 428

Human Alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) AssayLite Antibody (PerCP Conjugate)
12011-05071	AssayPro	150 ug	EUR 471

Shear wave speeds were, respectively, 3.13 ± 1.20 (m/s) for 300 Hz, 4.28 ± 1.36 (m/s) for 400 Hz, and 5.07 ± 1.45 (m/s) for 500 Hz for zebrafish 1 in a region of interest (ROI) which covered the central body. The shear wave speed dispersions were similar for four zebrafish and shear wave speeds ranged between 2.5 (m/s) and 5 (m/s) from 300 Hz to 500 Hz. The experimental setup and testing for a zebrafish lasted less than three minutes.

All tested zebrafish were alive after testing. ZUVE is safe, fast, and noninvasive, making the testing of elastic properties of zebrafish feasible.